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NameInstitutione-mail (optional)
Matt LewisDepartment of Pathology
University of Liverpool
m.lewis@liv.ac.uk

BrdU incorporation for keratinocyte raft cultures

Incubate rafts with BrdU (100 microgram per ml) for 12 hours prior to harvesting.

Detect incorporated BrdU with antibodies (Zymed 18-0103) and standard immunohistochemical techniques (with high temperature antigen retrieval to open up the DNA)

BrdU = Sigma B9285

Make stocks of BrdU at 10mg/ml in H2O, filter sterilise and store in working aliquots (eg. 200 microlitres) at 20C.

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