| Name | Institution | e-mail (optional) |
| Matt Lewis | Department of Pathology University of Liverpool |
m.lewis@liv.ac.uk |
BrdU incorporation for keratinocyte raft cultures
Incubate rafts with BrdU (100 microgram per ml) for 12 hours prior to harvesting.
Incubate rafts with BrdU (100 microgram per ml) for 12 hours prior to harvesting.
Detect incorporated BrdU with antibodies (Zymed 18-0103) and standard immunohistochemical techniques (with high temperature antigen retrieval to open up the DNA)
BrdU = Sigma B9285
Make stocks of BrdU at 10mg/ml in H2O, filter sterilise and store in working aliquots (eg. 200 microlitres) at ½20C.