NameInstitutione-mail (optional)
Matt LewisDepartment of Pathology
University of Liverpool

BrdU incorporation for keratinocyte raft cultures

Incubate rafts with BrdU (100 microgram per ml) for 12 hours prior to harvesting.

Detect incorporated BrdU with antibodies (Zymed 18-0103) and standard immunohistochemical techniques (with high temperature antigen retrieval to open up the DNA)

BrdU = Sigma B9285

Make stocks of BrdU at 10mg/ml in H2O, filter sterilise and store in working aliquots (eg. 200 microlitres) at �20C.